biotinylated goat anti mouse il 17f  (R&D Systems)

Journal: Immunity

Article Title: Differential roles of interleukin-17A and -17F in host defense against mucoepithelial bacterial infection and allergic responses.

doi: 10.1016/j.immuni.2008.11.009

Figure Lengend Snippet: Figure 1. IL-17F Contributes to the Devel- opment of Spontaneous Autoimmune Arthritis in Il1rn/ Mice (A) Profiles of intracellular IL-17F, IL-17A, and IFN-g expression in LN cells from wild-type and arthritic Il1rn/ mice stimulated with PMA and ionomycin in vitro. (B) Profiles of intracellular IL-17F, IL-17A, and IFN-g expression in cells from the ankle joints of wild-type and arthritic Il1rn/ mice stimulated with PMA and ionomycin. (C) Expression of IL-17A and IL-17F mRNA in the joints of arthritic Il1rn/ mice. (D) Arthritis incidence and severity scores in Il1rn/

Article Snippet: After washing with permeabilization buffer (0.1% saponin [Sigma] in staining buffer), cells were incubated with PE-anti-mouse IFN-g mAbs (XMG1.2; BD PharMingen), PE-anti-mouse IL-17A mAbs (TC11-18H10; BD PharMingen), or goat anti-mouse IL-17F polyclonal Abs (AF2057 or BAF2057; R&D systems).

Techniques: Expressing, In Vitro

Journal: Immunity

Article Title: Differential roles of interleukin-17A and -17F in host defense against mucoepithelial bacterial infection and allergic responses.

doi: 10.1016/j.immuni.2008.11.009

Figure Lengend Snippet: Figure 3. IL-17F and IL-17A Are Required for the Protection against C. rodentium Infection Wild-type, Il17f/, Il17a/, and Il17a/Il17f/ mice were orally infected with 2 3 108 CFU of C. rodentium, and the colons and spleens were harvested at the indicated time points after infection. (A) C. rodentium CFUs in colon homogenates (n = 10–16/group). Data show pooled results from two or three independent experiments. (B) Visualization of C. rodentium in the distal colon 14 days after oral infection (top, 403; bottom, 1203). Data are representative of four to six mice for each group. (C and D) Colon weight (C) and spleen weight (D) after oral infection as shown in (A). Data show pooled results from two or three independent experiments (n = 10–16/group). (E and F) Histopathology (E) and crypt length (F) in the distal colon 14 days after oral infection (H&E, 403). Data are pooled from two or three independent exper- iments (uninfected, n = 3/group; day 14, n = 20–23/group). In (F), white bars represent uninfected mice, and black bars represent day 14 mice. * p < 0.05, ** p < 0.01, and *** p < 0.001 versus wild-type mice. Data represent means ± SEM in (A) and (C)–(F).

Article Snippet: After washing with permeabilization buffer (0.1% saponin [Sigma] in staining buffer), cells were incubated with PE-anti-mouse IFN-g mAbs (XMG1.2; BD PharMingen), PE-anti-mouse IL-17A mAbs (TC11-18H10; BD PharMingen), or goat anti-mouse IL-17F polyclonal Abs (AF2057 or BAF2057; R&D systems).

Techniques: Infection, Histopathology

Journal: Immunity

Article Title: Differential roles of interleukin-17A and -17F in host defense against mucoepithelial bacterial infection and allergic responses.

doi: 10.1016/j.immuni.2008.11.009

Figure Lengend Snippet: Figure 4. IL-17F and IL-17A Are Required for the Induction of b-Defensin Expression during C. rodentium Infection (A) The expression of inflammatory mediators in the colon 14 days after infection with C. rodentium was determined with semiquantitative RT-PCR. (B) The expression of antimicrobial peptide in the colon 14 days after infection with C. rodentium was determined with real-time RT-PCR. Data represent the means ± SEM. The RNA sample was pooled from six to eight mice for each group. All data are representative of three independent experiments.

Article Snippet: After washing with permeabilization buffer (0.1% saponin [Sigma] in staining buffer), cells were incubated with PE-anti-mouse IFN-g mAbs (XMG1.2; BD PharMingen), PE-anti-mouse IL-17A mAbs (TC11-18H10; BD PharMingen), or goat anti-mouse IL-17F polyclonal Abs (AF2057 or BAF2057; R&D systems).

Techniques: Expressing, Infection, Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR

Journal: Immunity

Article Title: Differential roles of interleukin-17A and -17F in host defense against mucoepithelial bacterial infection and allergic responses.

doi: 10.1016/j.immuni.2008.11.009

Figure Lengend Snippet: Figure 5. IL-17F and IL-17A Are Produced by Different Cells (A) Colon, small intestine, and peripheral LNs from wild-type mice were analyzed for IL-17A and IL-17F mRNA expression with real-time RT-PCR. Expression in LN cells was defined as 1. (B) The expression of IL-17A and IL-17F in the colons of mice 7 and 14 days after infection with C. rodentium was determined with real-time RT-PCR. The RNA sample was a pool of samples from four to six mice for each group. The expression in uninfected wild-type mice was defined as 1. (C and D) Profiles of intracellular IL-17F, IL-17A, and IFN-g expression in colonic PMA- and ionomycin-stimulated lymphocytes from uninfected mice (C) or mice 14 days after infection with C. rodentium (D). (E and F) The colons and MLNs of C57BL/6J wild-type and Rag2/ mice 7 days after infection with C. rodentium were analyzed for IL-17A and IL-17F mRNA expression with real-time RT-PCRs (E) (n = 5–6/group). The expression in wild-type colon was defined as 1. The expression of these cytokines in the colon and MLNs of Rag2/ mice was determined as a percentage of the expression in wild-type mice (F). (G) Whole colons of uninfected wild-type, Rag2/, and Il17a/Il17f/ mice were cultured for 24 hr in the presence or absence of 20 ng/ml IL-23. The concen- trations of IL-17A or IL-17F in supernatant were determined by ELISAs and were normalized to total protein content for each sample (n = 5–8/group). Similar results were also observed in C.B-17 SCID mice. (H and I) Splenocytes (5 3 105 cells) (H) or MLNs (1.5 3 105 cells) (I) of wild-type and Rag2/ mice were cultured in 24- or 48-well plates, respectively, in the presence or absence of 5 mg/ml LPS and 20 ng/ml IL-23 for 72 hr, and IL-17A and IL-17F amounts in culture supernatants were determined with ELISA. (J) Colonic epithelial (CD45 and high FSC and SSC; gates R1 and R3) cells and intraepithelial immune cells (CD45+; gate R2) were isolated from the colons of uninfected wild-type mice with flow cytometry, and the expression of IL-17F and IL-17A was examined with RT-PCR.

Article Snippet: After washing with permeabilization buffer (0.1% saponin [Sigma] in staining buffer), cells were incubated with PE-anti-mouse IFN-g mAbs (XMG1.2; BD PharMingen), PE-anti-mouse IL-17A mAbs (TC11-18H10; BD PharMingen), or goat anti-mouse IL-17F polyclonal Abs (AF2057 or BAF2057; R&D systems).

Techniques: Produced, Expressing, Quantitative RT-PCR, Infection, Cell Culture, Enzyme-linked Immunosorbent Assay, Isolation, Cytometry, Reverse Transcription Polymerase Chain Reaction

Journal: Immunity

Article Title: Differential roles of interleukin-17A and -17F in host defense against mucoepithelial bacterial infection and allergic responses.

doi: 10.1016/j.immuni.2008.11.009

Figure Lengend Snippet: Figure 6. IL-17RA and IL-17RC Show Different Tissue Distributions (A and B) The expression of IL-17RA and IL-17RC in tissues from 129/Ola 3 C57BL/6J wild-type mice (A), and different cell lines (B) were determined with real- time RT-PCR. (C) The expression of IL-17RA, IL-17RC, and Act1 in different cell populations obtained by MACS sorting was determined with RT-PCR. (D) Peritoneal macrophages were stimulated for 24 hr with 5–250 ng/ml IL-17A or IL-17F, or 10–100 ng/ml LPS, and IL-6 amounts in the culture supernatants were determined with ELISAs. (E) CD4+ T cells from C57BL/6J mice obtained by MACS sorting were stimulated for 48 hr with 5–250 ng/ml IL-17A or IL-17F, and CCL2 in the culture supernatants was determined with Bio-Plex suspension array system (Bio-Rad). (F) The expression of lipocalin 2 and b-defensin 3 in colonic epithelial cell line (CMT93) stimulated for 6 hr with 5–250 ng/ml IL-17A or IL-17F individually, or with combination of 50–250 ng/ml IL-17A and IL-17F, was determined with real-time RT-PCR. (G–I) Colonic epithelial cell line (CMT93) (G), peritoneal macrophages from C3H/HeJ mice (H), or CD4+ T cells from C57BL/6J mice (I) were stimulated for 24 hr (G and H) or 48 hr (I) with 50 ng/ml IL-17A or IL-17F individually, or with a combination of 50 ng/ml IL-17A and IL-17F. IL-1b, IL-9, GM-CSF, CCL3, or CXCL1 in the culture supernatants were determined with the Bio-Plex suspension array system (Bio-Rad). ND denotes not detected. * p < 0.05, ** p < 0.01, and *** p < 0.001 versus medium alone. All data represent means ± SEM and are representative of three independent experiments.

Article Snippet: After washing with permeabilization buffer (0.1% saponin [Sigma] in staining buffer), cells were incubated with PE-anti-mouse IFN-g mAbs (XMG1.2; BD PharMingen), PE-anti-mouse IL-17A mAbs (TC11-18H10; BD PharMingen), or goat anti-mouse IL-17F polyclonal Abs (AF2057 or BAF2057; R&D systems).

Techniques: Expressing, Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Suspension